Description:
SCLAVO APTT-S Kit is also used in assays which quantitate these factors and in monitoring heparin therapy1, 2. Antiphospholipid antibodies and non-specific inhibitors (lupus-like anticoagulants) may cause prolonged aPTT values. However, this effect is recognized as being related to the inhibitor concentration, and occurs inconsistently. In the basic screening test, the activated partial thromboplastin time indirectly measures the formation of thrombin by its action on fibrinogen forming the fibrin clot. In the test, citrated test plasma is mixed with aPTT reagent for a specified period of time (typically 5 minutes) at 37°C followed by the addition of pre- warmed (37°C) calcium chloride (0.025M). Timing is begun from the time of addition of calcium chloride. The time required for clot formation is the activated partial thromboplastin time (aPTT). Clot detection can be by mechanical, manual (tilt tube), or photo-optical (e.g., Sysmex CA1500) measurement.
